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Official sources said that 250 chicken died on Wednesday morning at a private poultry farm, under the Maheshtala municipality, in South 24-Parganas.
District health officials said that there had been no instance of avian flu in the area earlier. Blood samples have been collected from the farm situated at Benerpukur, health officials said.
Meanwhile, culling operations continued in Budge Budge, from where a large number of chicken deaths were earlier reported.
In North 24-Parganas, where bird flu has not been officially declared, culling has been taken up in Baduria, Swarupnagar and Habra blocks on symptomatic evidence, District Magistrate Prabhat Mitra said.
Culling in backyard poultry and in poultry farms has been taken up in a radius of five km of the affected areas in the three blocks, the DM said. Over 4,000 chicken deaths had been reported from a private farm in Baduria on Tuesday.
With avian deaths reported from the district, Kolkata is now ringed by bird flu affected districts - South and North 24-Parganas, Howrah and Hooghly.

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