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Totti missed the first leg last week with a thigh problem and there had been conflicting reports over the weekend about the striker's availability for the Old Trafford leg, with Roma trailing 2-0.
The forward, however, was not included in a squad list published on the club’s Web site.
Roma boss Luciano Spalletti had said after Saturday's 3-2 win over Genoa that it might be better for Totti to miss the United game and fully recover for next weekend's league clash at Udinese.
Second-placed Roma are four points behind Inter Milan in the race for the Italian title with the domestic championship now taking priority over the Champions League given the unlikelihood of overhauling United.
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