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Authorities of the two districts confirmed the spread of avian flu and said that culling operation would begin on Monday.
West Midnapore District Magistrate N S Nigam said samples of chicken sent from Chakbelcha village in Debra block of Kharagpur subdivision had tested positive.
In South 24 Parganas the disease tested positive in Budge Budge, District Magistrate Sanghamitra Ghosh said.
Nigam said 40 teams would start culling operations in 5 km radius of Chakbelcha on Monday.
Meanwhile, birdflu spread to Baro Khalasamari at Mathabhanga block in affected Coochbehar district, where the disease had hit four villages in Dinhata bocks I and II.
In the affected district of Howrah, avian flu spread to Panchla in addition to Sankrail. Reports from the district said a fox and other birds like falcon were found dead there.
About 15.75 lakh hens and cocks have already been culled so far out of the target of 22 lakh in 11 districts and 1.25 lakh had died, West Bengal Animal Resources Development Minister Anisur Rehman said during the day.
"The rest of the birds would be culled tomorrow," he said.
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